EC Number |
General Stability |
Reference |
---|
3.4.19.3 | 2-mercaptoethanol and EDTA stabilize |
95283, 95290, 95292 |
3.4.19.3 | addition of 1% bovine serum albumin to the purified enzyme improves stability with 88% of the activity remaining after 4 days at 4°C and 93% of the activity remaining after 21 days at -80°C |
95299 |
3.4.19.3 | enzyme solubilized by sodium deoxycholate and Triton X-100, -20°C or 4°C, 24 h, more than 50% loss of activity |
95299 |
3.4.19.3 | freezing and thawing, 67% of the activity is recovered after one cycle, 70% loss of activity after 5 cycles |
95294 |
3.4.19.3 | subunit interactions play an important role in stabilizing the enzyme in addition to the intrinsic enhanced stability of its monomer |
651099 |
3.4.19.3 | trypsin solubilized enzyme, no loss of activity after 14 days at 4°C, 20% loss of activity after 14 days at -20°C |
95299 |
3.4.19.3 | wild-type and mutant enzyme C142S/C188S, the anomalous high stability of the hyperthermophilic enzyme originates from the unusually slow rate of unfolding reaction during treatment with guanidine-hydrochloride |
95297 |