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EC Number
Activating Compound
Commentary
Reference
3.5.5.5
2-mercaptoethanol
1.4fold at 50 mM, 1.6fold activation at 100 mM
652959
3.5.5.5
2-mercaptoethanol
activation
32502
,
32503
3.5.5.5
dithiothreitol
activation
32502
,
32503
3.5.5.5
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expression is induced by growing the strain in the presence of different nitriles and /or complex or inorganic nitrogen sources. The highest nitrile hydrolysing activity is observed with cells grown with 2-cyanopyridine and NaNO3
701254
3.5.5.5
phenylacetonitrile
absolutely required as growth nitrogen source substrate for induction of the enzyme, cannot be substituted by ammonia or epsilon-caprolactam
652959
3.5.5.5
Sodium hydrosulfide
activation
32503
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