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EC Number
Activating Compound
Commentary
Reference
3.4.22.32
2,3-dimercapto-1-propanol
not as effective in activation as cysteine
30285
3.4.22.32
cysteine
inactive unless treated with
30285
3.4.22.32
DTT
not as effective in activation as cysteine
30285
3.4.22.32
Guanidine-HCl
at 2 mM
680551
3.4.22.32
more
bromelain activator solution consists of 0.02 M EDTA and 0.05 M L-cysteine, pH 8.0
709557
3.4.22.32
more
like ficin and unlike papain, bromelain does not possess a carboxyl group capable of enhancing the rate of reaction of the active-centre thiol group with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole
30291
3.4.22.32
Thioglycol
not as effective in activation as cysteine
30285
3.4.22.32
Urea
at 5 M and 8-9 M
680551
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