EC Number |
Activating Compound |
Reference |
---|
3.4.21.4 | alkylbenzene sulfonate |
1% v/v, 257% of initial activity |
731844 |
3.4.21.4 | caerulein |
trypsinogen activation to trypsin occurs with 10 nM caerulein, trypsinogen is activated to reach a trypsin level of 6 nM following 10 nM caerulein induction for 1 h |
696328 |
3.4.21.4 | dithiothreitol |
addition of 2 mM dithiothreitol greatly enhances the tryptic activity |
698277 |
3.4.21.4 | EDTA |
increase in activation of pro-matrix metalloproteinase-2 |
669859 |
3.4.21.4 | enterokinase |
trypsin is activated with 100 U/l enterokinase |
698513 |
3.4.21.4 | enterokinase |
trypsinogens (0.0002 mM) are incubated at room temperature with 6-200 ng enterokinase in 0.1 M Tris (pH 8), containing 5 mM CaCl2 and 0.01% bovine serum albumin for 10-60 min |
700478 |
3.4.21.4 | enteropeptidase |
trypsinogen is activated through the cleavage of a peptide Val-Asp-Asp-Asp-Asp-Lys-Ile located on the N-terminus of the protein, the heptapetide is cleaved autocatalytically by its physiological activator, enteropeptidase |
699194 |
3.4.21.4 | methyl-alpha-D-galactoside |
1.2fold enhancement of activity at 0.2 M |
680742 |
3.4.21.4 | methyl-alpha-D-mannoside |
1.4fold enhancement of activity at 0.2 M |
680742 |
3.4.21.4 | more |
activity is not enhanced in 0.2 M lactose |
680742 |