2.8.1.11 | IscS protein |
IscS, a three-domain rhodanese-like protein, specifically interacts with YnjE for the formation of the persulfide group on YnjE and enhances the reaction in an in vitro system, consisting of MPT synthase, MoeB, Mg-ATP, IscS, L-cysteine, and YnjE. It also interacts with MoeB. The role of YnjE is to make the sulfur transfer from IscS for Moco biosynthesis more specific, since IscS is involved in a variety of different sulfur transfer reactions in the cell. IscS is the preferred sulfur donor for YnjE in vivo |
722726 |
2.8.1.11 | YnjE protein |
specifically interacts with IscS for the formation of the persulfide group on YnjE and enhances the reaction in an in vitro system, consisting of MPT synthase, MoeB, Mg-ATP, IscS, L-cysteine, and YnjE. Best activating in a ratio of 1:2 with MPT, is inhibitory at ratio 1:10. It also interacts with MoeB. The role of YnjE is to make the sulfur transfer from IscS for Moco biosynthesis more specific, since IscS is involved in a variety of different sulfur transfer reactions in the cell. IscS is the preferred sulfur donor for YnjE in vivo. Nevertheless YnjE is not a real enhancer of L-cysteine desulfurase activity, but rather leads to a better rate of conversion of cPMP to MPT. YnjE occurs in the cytosol and the periplasm |
722726 |