EC Number |
Activating Compound |
Reference |
---|
2.7.7.59 | (S)-N-dansyl-Tyr-Val-alpha-hydroxyglycine |
plays a central role in uridylylation reaction, its required concentration for maximal uridylylation depends on the present cation |
674326 |
2.7.7.59 | 2-oxoglutarate |
- |
702974 |
2.7.7.59 | 2-oxoglutarate |
at low concentration, required for PII uridylylation |
643544 |
2.7.7.59 | 2-oxoglutarate |
maximum uridylylation rates for both PII proteins GlnB and GlnK are observed in the presence of about 2 mM 2-oxoglutarate and 200500 microM ATP. Uridylylation of both GlnB and GlnK responds to 2-oxoglutarate levels, but only GlnB responds effectively to variation on ADP/ATP ratio |
721459 |
2.7.7.59 | 2-oxoglutarate |
required compound, uridylylation assay with 5 mM 2-oxoglutarate |
676989 |
2.7.7.59 | ADP |
or ATP, absolutely required for in vitro uridylylation of PII proteins GlnB and GlnK. Maximum uridylylation rates for both proteins are observed in the presence of about 2 mM 2-oxoglutarate and 200-500 microM ATP. Uridylylation of both GlnB and GlnK responds to 2-oxoglutarate levels, but only GlnB responds effectively to variation on ADP/ATP ratio |
721459 |
2.7.7.59 | AMP |
may partially replace ATP or ADP |
721459 |
2.7.7.59 | ATP |
- |
702974 |
2.7.7.59 | ATP |
or ADP, absolutely required for in vitro uridylylation of PII proteins GlnB and GlnK. Maximum uridylylation rates for both proteins are observed in the presence of about 2 mM 2-oxoglutarate and 200-500 microM ATP. Uridylylation of both GlnB and GlnK responds to 2-oxoglutarate levels, but only GlnB responds effectively to variation on ADP/ATP ratio |
721459 |
2.7.7.59 | ATP |
required compound, uridylylation assay with 0.2 mM ATP |
676989 |