EC Number   |
Activating Compound |
Reference |
|---|
    1.14.14.19 | CYP21 |
direct molecular interactions, with electrostatic interactions playing a crucial role, between steroidogenic enzymes CYP17 and CYP21, EC 1.14.99.10, that are localized in endoplasmic reticulum membranes of adrenal cortex and involved in biosynthesis of corticosteroid hormones. The interaction in vitro reduces the catalytic activities of both enzymes at high ionic strength, i.e. 300 mM NaCl, while it increases activity at low ionic strength, i.e. 100 mM NaCl, overview |
726923 |
| 1.14.14.19 | cytochrome b5 |
- |
390122 |
| 1.14.14.19 | cytochrome b5 |
above pH 6.3-6.5 stimulates 17alpha-hydrolase and c17,20-lyase activity |
390139 |
| 1.14.14.19 | cytochrome b5 |
acts as allosteric facilitator but not as electron donor, increases 17,20-lyase activity |
390120 |
| 1.14.14.19 | cytochrome b5 |
expression of cytochrome b5 in the zona reticularis, at the onset of adrenarche, increases along with the acyl-carbon bond cleavage activity of CYP17, cytochrome plays a regulatory role for CYP17 activity |
716053 |
| 1.14.14.19 | cytochrome b5 |
recombinant human microsomal wild-type protein and mutants E48Q, E49Q, and V50S, enzyme interaction analysis by NMR, overview. Substrate-modulated interactions of cytochrome P450 17A1 and cytochrome b5, by reversible binding, involving enzyme anionic residues Glu48 or Glu49 and corresponding cationic CYP17A1 residues Arg347, Arg358, and Arg449. The CYP17A1/b5 interaction is stronger when the hydroxylase substrate pregnenolone is present in the CYP17A1 active site than when the lyase substrate 17alpha-hydroxypregnenolone is in the active site |
727947 |
| 1.14.14.19 | cytochrome b5 |
specific stimulation of 17,20-lyase activity of enzyme, up to 10fold increase in lyase activity, while 17alpha-hydroxylase activity is little affected |
672187 |
| 1.14.14.19 | more |
cytochrome B5 does not affect the 17alpha-hydroxylase activity |
686364 |
| 1.14.14.19 | more |
not activating: antiepileptic drugs valproic acid, carbamazepine, topiramate, lamotrigine |
673400 |
| 1.14.14.19 | phosphatidylcholine |
incorporation of P450 into liposomal membranes composed of phosphatidylcholine increases activity |
390134 |
