EC Number |
Activating Compound |
Reference |
---|
1.14.13.81 | ascorbate |
prevents degradation of tetrapyrroles by reactive oxygen species |
636329 |
1.14.13.81 | ATP |
requirement for reconstituted system |
636333 |
1.14.13.81 | catalase |
prevents degradation of tetrapyrroles by reactive oxygen species |
636329 |
1.14.13.81 | isoascorbate |
prevents degradation of tetrapyrroles by reactive oxygen species |
636329 |
1.14.13.81 | S-adenosyl-L-methionine |
- |
636332 |
1.14.13.81 | S-adenosyl-L-methionine |
1 mM, absolute requirement when magnesium-protoporphyrin IX is used as substrate |
636333 |
1.14.13.81 | XanL |
required by the enzyme, found associated with the membrane and protein Ycf54 |
744871 |
1.14.13.81 | Ycf54 |
essential for cyclase activity and/or stability forming a complex with other oxidative cyclase components Sll1214 and Sll1874 |
727915 |
1.14.13.81 | Ycf54 |
identified based upon strong association of Ycf54 with XanL. Active Ycf54 is membrane localized. The conserved chloroplast polypeptide Ycf54 is involved in the MPEC-enzyme catalyzed reaction. Sequencing, recombinant expression as His-tagged protein, and purification, UniProt ID F2D891 |
744871 |
1.14.13.81 | Ycf54 protein |
the Ycf54 protein forms a complex with the component of the oxidative cyclase, Sll1214 (CycI, gene slr1780). Partial inactivation of the ycf54 gene leads to Chl deficiency in cyanobacteria and plants, and the complete deletion of the ycf54 gene in the cyanobacterium Synechocystis 6803 results accumulation of huge concentrations of the cyclase substrate MgPME together with 3-formyl MgPME, in the DELTAycf54 strain. The Ycf54 protein is important, but not essential, for activity of the oxidative cyclase |
744998 |