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EC Number Activating Compound Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.1.99.37more the low coenzyme NAD-dependent activity of MDH with C1-C4 primary alcohols is strongly stimulated by Bacillus methanolicus protein ACT, in presence of NAD(H) cofactor and Mg2+-ions. In the deduced ACT amino acid sequence, the highly conserved amino acid sequence motif typical of Nudix hydrolase proteins is present. MDH activation by ACT involves hydrolytic removal of the nicotinamide mononucleotide NMN(H) moiety of the NAD(H) cofactor of MDH, changing its Ping-Pong type of reaction mechanism into a ternary complex reaction mechanism 656074
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