EC Number   |
Activating Compound  |
Reference |
|---|
   3.4.22.33 | 2-mercaptoethanol |
highest activity at 96 mM, at 37°C and pH 7.0 |
682716 |
| 3.4.22.33 | dithiothreitol |
highest activity at 2.5 mM, at pH 7.0 and 37°C |
682716 |
| 3.4.22.33 | EDTA |
28% increase of activity at 2 mM |
682716 |
| 3.4.22.33 | EDTA |
activates about 2.5fold |
753642 |
| 3.4.22.33 | more |
high-pressure treatment of fruit bromelain with 300 MPa, at 20°C for 20 min: high pressure processing (HPP) affects the fibrinolytic activity of fruit bromelain in vivo, overview. Compared with untreated fruit bromelain, the fibrinolytic activity of high-pressure processing-treated fruit bromelain is obviously increased which might be attributed to the increase of alpha-helix and the exposure of tryptophan residues and tyrosine residues, overview. High-pressure processing-treated fruit bromelain shows an increase (7.67%) in alpha-helix and its fluorescence intensities are increased by 42.5% and 78.7% at the excitation wavelength of 295 nm and 280 nm, respectively |
754304 |
| 3.4.22.33 | more |
no effect on activity by EDTA |
753642 |
| 3.4.22.33 | Urea |
activity decreases in the presence of 4 M urea and reaches the activity of the control again at 8 M when using casein as a substrate |
682716 |
