EC Number |
Activating Compound |
Reference |
---|
2.3.1.54 | Ferredoxin |
- |
487218, 487219, 487222 |
2.3.1.54 | Ferredoxin |
methylviologen can replace ferredoxin |
487222 |
2.3.1.54 | Ferredoxin |
reactivation of inactive enzyme requires reduced ferredoxin, Fe2+-dithiol or Co2+-mercaptoethanol complexes can substitute for ferredoxin |
487218 |
2.3.1.54 | more |
conversion into the active form is carried out by an activating system containing a flavodoxin system and an activating enzyme (pyruvate format lyase-activase, EC 1.97.1.4) |
663000 |
2.3.1.54 | NADPH |
more effective for activation than NADPH, 0.02 mM NADPH activates over 80% inactive enzyme in cell-free extracts |
663000 |
2.3.1.54 | oxamate |
- |
487222 |
2.3.1.54 | oxamate |
obligatory component of the activation reaction |
487221, 487239 |
2.3.1.54 | pyruvate |
obligatory component of the activation reaction |
487221, 487239 |
2.3.1.54 | pyruvate formate lyase activating enzyme |
activates the enzyme by forming a glycine radical, cf. EC 1.97.1.4 |
744943 |
2.3.1.54 | pyruvate formate-lyase activating enzyme |
converts the enzyme to its catalytically active state by generating a stable glycyl radical in the active site at G734 |
757586 |