3.4.22.45 A387E no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching 698120 3.4.22.45 C16A substitution mutants introduced into amino-proximal region of helper component protease HC-Pro, vector transmission abolished 683105 3.4.22.45 C16AC20A double mutant generated, vector transmission abolished 683105 3.4.22.45 C20A no effects on vector transmission 683105 3.4.22.45 C20R no effects on vector transmission, randomized substitution 683105 3.4.22.45 C291S inactive in aphid transmission 137295 3.4.22.45 C305S active in aphid transmission, similar to wild-type 137295 3.4.22.45 C344A/H417A the mutant shows abolished self-cleavage 732424 3.4.22.45 C390W no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching 698120 3.4.22.45 C46A vector transmission abolished 683105