3.1.1.89	K38A	mutation does not abolish nuclear localization of enzyme	714919	
3.1.1.89	additional information	construction of deletion mutants and expression in HeLa cells. Domain 1 of the enzyme extends from amino acids 1 to 233, domain 2 from amino acids 234 to 284 and domain 3 includes amino acids 285 to 378. Domain 1 of PME-1 does not migrate into the nucleus and domain 3 shows a speckled cytoplasmic distribution, whereas domain 2 is targeted to the nucleus	714919	
3.1.1.89	R271A	mutation abolishes nuclear localization of enzyme	714919	
3.1.1.89	R37A	mutation does not abolish nuclear localization of enzyme	714919	
3.1.1.89	R39A	mutation does not abolish nuclear localization of enzyme	714919	
3.1.1.89	S156A	mutation abolishes the methylesterase activity, residue S156 is a functional active site serine	714919