2.5.1.30 A79F mutant enzyme subunit-I(wild-type)/subunit-B(A79F). Comparable kinetic constants with those of the wild-type enzyme. Significantly different pattern of product distribution from that of the wild type. The major product is hexaprenyl diphosphate 702201 2.5.1.30 A79L mutant enzyme subunit-I(wild-type)/subunit-B(A79L). Comparable kinetic constants with those of the wild-type enzyme. Significantly different pattern of product distribution from that of the wild type. The major product is hexaprenyl diphosphate 702201 2.5.1.30 A79Y single replacement to the aromatic residue at the fourth or the fifth position before the first aspartate-rich motif (FARM), mainly yields a C20 product 637630 2.5.1.30 D73A mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type 637629 2.5.1.30 D97A mutant enzyme subunit-I(D97A)/subunit-II(wild-type), major products: geranylgeranyl diphosphate, farnesylgeranyl diphosphate, hexaprenyl diphosphate, heptaprenyl diphosphate 702201 2.5.1.30 D97A reaction products have marked differences in chain length distribution from the wild-type enzyme. D97A produces larger amounts of shorter chain prenyl diphosphates 637629 2.5.1.30 D97A/A79F mutant enzyme subunit-I(97A)/subunit-II(A79F) forms exclusively geranylgeranyl diphosphate 702201 2.5.1.30 D97A/A79L mutant enzyme subunit-I(D97A)/subunit-II(A79L), the final product is farnesylgeranyl diphosphate, increase in the production of geranylgeranyl diphosphate 702201 2.5.1.30 E128V mutant shows similar Km-value for farnesyl diphosphate and Vmax-value compared to the wild-type 637629 2.5.1.30 I76G can catalyze condensations of isopentenyl diphosphate beyond the native chain length of C35. With farnesyl diphosphate as allylic substrate the mutant enzyme largely produces (all-trans)-octaprenyl diphosphate (C40, 21%) with a small amount of solanesyl diphosphate (C45, 5.3%). With geranylgeranyl diphosphate as allylic substrate the mutant enzyme yields solanesyl diphosphate (33%) as the main product with a large amount of (all-trans)-octaprenyl diphosphate (21%) 637630