2.3.1.250	C187A	site-directed mutagenesis, increase in Wnt signaling activity of about 1.5fold upon expression of PORCNC187A compared to wild-type PORCN	734745	
2.3.1.250	C375X	site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 resi­due, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant	734745	
2.3.1.250	C381X	site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 resi­due, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant	734745	
2.3.1.250	C385X	site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 resi­due, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant	734745	
2.3.1.250	H341A	site-directed mutagenesis, catalytic active site mutant, Wnt signaling inactive mutant	734194	
2.3.1.250	H341A	site-directed mutagenesis, catalytically inactive mutant	734745	
2.3.1.250	H341A	site-directed mutagenesis, the mutation reduces Wnt signaling relative to wild-type PORCN	734745	
2.3.1.250	H341D	site-directed mutagenesis, catalytically inactive mutant	733699	
2.3.1.250	H341Q	site-directed mutagenesis, catalytically inactive mutant	733699	
2.3.1.250	additional information	generation of three different deletion mutants of the enzyme lacking residues 1-34, 1-66, or 1-110 (DELTA34, DELTA66, and DELTA110), the mutants show affeced N-glycosylation of Wingless protein, because the mutant enzymes cannot bind to Wingless, overview	733769