1.7.1.17	F117A	the mutant shows 208% activity with Methyl Red and 176% activity with NADH compared to the wild type enzyme	765245	
1.7.1.17	F161A	inactive	765245	
1.7.1.17	F162A	site-directed mutagenesis	742834	
1.7.1.17	G140F	inactive	765245	
1.7.1.17	G141F	inactive	765245	
1.7.1.17	H143A	the mutant shows 19% activity with Methyl Red and 76% activity with NADH compared to the wild type enzyme	765245	
1.7.1.17	additional information	deletion of the azoR gene in Escherichia coli strain MG1655 has no strong influence on the 7NCCA reduction of the cells even though the pure enzyme displays a clear nitroreductase activity with this compound	743185	
1.7.1.17	N121Q	115% of wild-type activity	-, 744201	
1.7.1.17	N96A	the mutant shows 9% activity with Methyl Red and 37% activity with NADH compared to the wild type enzyme	765245	
1.7.1.17	R139A	the mutant shows 63% activity with Methyl Red and 78% activity with NADH compared to the wild type enzyme	765245