1.14.12.22 degradation constitutive expression of terminal oxygenase subunit in Rhizobium tropici. In water-cultured siratro rhizospheres inoculated with the recombinant strain, 48% of the initial 25 microg dibenzofuran is removed within 3 days, and similar results are obtained in soil-cultured siratro rhizosperes using sterile vermiculite. In non-sterile field soils, the inoculated recombinant strain can grow on the siratro root in all soils tested, except for wet paddy field -, 707774 1.14.12.22 F275A mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene 707776 1.14.12.22 F275I mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene 707776 1.14.12.22 F275L mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene 707776 1.14.12.22 F275V mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene 707776 1.14.12.22 F275W mutation in oxygenase component. Decrease in activity with carbazole, wild-type like activity with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene 707776 1.14.12.22 F275W the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme 741682 1.14.12.22 F329A mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene 707776 1.14.12.22 F329I mutation in oxygenase component. Strong decrease in activity with all substrates tested 707776 1.14.12.22 F329L mutation in oxygenase component. Strong decrease in activity with all substrates tested 707776