1.14.11.69	D191A	site-directed mutagenesis, the mutant shows about 95%reduced activity with H3K9me3 compared to wild-type, and no activity with H3K36me3	754871	
1.14.11.69	G133A	site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity	753371	
1.14.11.69	G138A	site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity	753371	
1.14.11.69	G165A	site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity	753371	
1.14.11.69	G170A	site-directed mutagenesis, the mutation in the catalytic domain abrogates enzyme activity	753371	
1.14.11.69	H195A	construction of a mutant dKDM4A in which a conserved amino acid in the iron-binding site is mutated to alanine, the mutant dKDM4A has no demethylation activity on histones H3K36me3 and H3K36me2	700209	
1.14.11.69	H195A	site-directed mutagenesis	724813	
1.14.11.69	H195A	site-directed mutagenesis, mutation of an Fe2+ binding residue, abolishes demethylase activity of dJMJD2(1)/CG15835	754921	
1.14.11.69	H235A	catalytically deficient mutant	-, 726071, 754922	
1.14.11.69	additional information	construction of a P-element insertion mutant of dKDMA4, the mutant is homozygous viable, the P element insertion elevates the bulk level of histone H3K36me3 in mutant embryos. Overexpression of dKDM4A seems to only lead to demethylation of histone H3K36, since the level of histone H3K9me3 and H3K4me2 remains unchanged	700209