1.1.1.274 A272G mutation increases Km and kcat compared to the wild-type enzyme 657308 1.1.1.274 F22Y isoenzyme A, similar kcat as wild-type 440307 1.1.1.274 F22Y mutation reduces Km and increases kcat by 50% compared to the wild-type enzyme 657308 1.1.1.274 F22Y/A272G increased activity compared to the wild-type enzyme, substrate inhibition at substrate concentrations above 17.5 mM 657308 1.1.1.274 F22Y/A272G isoenzyme A, reduced kcat for NADH 440311 1.1.1.274 F22Y/K232G/R235G/R238E/A272G isoenzyme A, increase in kcat for NADH 440311 1.1.1.274 F22Y/K232G/R235G/R238H/A272G isoenzyme A, increase in kcat for NADH 440311 1.1.1.274 F22Y/K232G/R235T/R238E/A272G isoenzyme A, 24fold increase in kcat for NADH 440311 1.1.1.274 F22Y/K232G/R238H/A272G isoenzyme A, increase in kcat forNADH 440311 1.1.1.274 F22Y/K232G/R238H/A272G mutant shows a higher activity with NADH compared to the wild-type enzyme 657308