5.3.2.6	A33D	the mutant shows highest enantioselectivity for the Michael-type addition of acetaldehyde to trans-beta nitrostyrene producing 4-nitro-3-phenylbutanal, compared to the wild type enzyme	748742	
5.3.2.6	A33E	the mutation significantly (4fold) improves the activity of the enzyme for the Michael-type addition of acetaldehyde to trans-beta nitrostyrene, compared to the wild type enzyme	748742	
5.3.2.6	alphaI52E	site-directed mutagenesis, active site mutant,the mutant shows improved trans-3-chloroacrylic acid dehalogenase activity with a 36fold increase in kcat/Km, largely due to a 110fold decrease in Km, and diminished 4-oxalocrotonate tautomerase activity. The negatively charged group may hinder the formation of the enolate intermediate and may contribute to a decrease in kcat	-, 718844	
5.3.2.6	alphaL8R	site-directed mutagenesis, active site mutant, the mutant shows improved trans-3-chloroacrylic acid dehalogenase activity with a 50fold increase in kcat/Km, primarily from an 8.8fold increase in kcat, and diminished 4-oxalocrotonate tautomerase activity with a 5fold decrease in kcat/Km. The increased CaaD activity of L8R-4-OT does not substantially diminish the original 4-OT activity	-, 718844	
5.3.2.6	alphaL8R/I52E	site-directed mutagenesis, active site mutant, the mutant shows improved trans-3-chloroacrylic acid dehalogenase activity with a 32fold increase in kcat/Km, largely due to a 23fold decrease in Km, and diminished 4-oxalocrotonate tautomerase activity with a 1700fold decrease in kcat/Km	-, 718844	
5.3.2.6	alphaR12A	site-directed mutagenesis, the mutant shows highly reduced catalytic efficiency compared to the wild-type hh4-OT	-, 718874	
5.3.2.6	alphaR40A	site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type hh4-OT	-, 718874	
5.3.2.6	betaP1A	site-directed mutagenesis, the mutant shows highly reduced catalytic efficiency compared to the wild-type hh4-OT	-, 718874	
5.3.2.6	betaR11A	site-directed mutagenesis, the mutant shows increased catalytic efficiency compared to the wild-type hh4-OT	-, 718874	
5.3.2.6	betaR39A	site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type hh4-OT	-, 718874