4.4.1.15	E295S	By site-directed mutagenesis. The Pseudomonas putida UW4 single mutant is constructed using pET30a (+) with the full-length ACC deaminase as the template	-, 694780	
4.4.1.15	E295S/L322T	The double mutant is constructed using the E295S mutant as the template.	-, 694780	
4.4.1.15	H80Q	site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme	730698	
4.4.1.15	Q77H	site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 30% activity with beta-chloro-D-alanine	730698	
4.4.1.15	S358E	By site-directed mutagenesis. The single mutants of the tomato enzyme are constructed using pET30 Xa/LIC with the full-length putative ACC deaminase as the template	694780	
4.4.1.15	S358E/T386L	The double mutant is constructed using an additional round of mutagenesis and with the S358E single mutant as the template	694780	
4.4.1.15	S78A	site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 20% activity with beta-chloro-D-alanine	730698	
4.4.1.15	T288E	site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme	730698	
4.4.1.15	T315L/T288E	site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme	730698	
4.4.1.15	Y261F	site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme	730698