4.3.3.6 D26A/K83A/K151A no formation of an internal chromophore with characteristic absorbance at 320 nm during reaction (observed with wild type protein) 724239 4.3.3.6 D99A mutant of Pdx1 (pyridoxal 5'-phosphate synthase subunit), 5% of the pyridoxal 5'-phosphate synthase activity compared to wild-type enzyme with L-glutamine as N-donor, 17.2% of the glutaminase activity compared to wild-type enzyme 714170 4.3.3.6 DELTA 270-301 mainly monomer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis 724999 4.3.3.6 DELTA 273-301 dodecamer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis 724999 4.3.3.6 DELTA 279-301 dodecamer, Pdx2 activation, reduced pyridoxal 5'-phosphate synthesis 724999 4.3.3.6 DELTA 287-301 dodecamer, Pdx2 activation, precipitates upon addition of glyceraldehyd 3-phosphate 724999 4.3.3.6 DELTA 293-301 behaviour like DELTA 287-301 724999 4.3.3.6 DELTA 295-301 behaviour like DELTA 287-301 724999 4.3.3.6 E105D Pdx1 726269 4.3.3.6 E116A activity as the wild-type protein 724219