4.1.3.36 D163A loss of the ligand binding ability 714260 4.1.3.36 D163E 14% 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity 714260 4.1.3.36 D163N no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity 714260 4.1.3.36 D185E reduced kcat 714273 4.1.3.36 D185G no activity 714273 4.1.3.36 D185N reduced kcat 714273 4.1.3.36 D192N no activity 714273 4.1.3.36 F270A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 728604 4.1.3.36 G156D no activity 714273 4.1.3.36 K89A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 728604