3.4.22.53	E504S	Ca2+ concentration required for half-maximal activity is 0.129 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme	644019	
3.4.22.53	K234W	Ca2+ concentration required for half-maximal activity is 0.159 mM compared to 0.242 mM for the wild-type enzyme	644019	
3.4.22.53	K234S	Ca2+ concentration required for half-maximal activity is 0.183 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme	644019	
3.4.22.53	K226S	Ca2+ concentration required for half-maximal activity is 0.226 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme	644019	
3.4.22.53	K230E	Ca2+ concentration required for half-maximal activity is 0.256 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme	644019	
3.4.22.53	K230S	Ca2+ concentration required for half-maximal activity is 0.261 mM compared to 0.242 mM for the wild-type enzyme	644019	
3.4.22.53	C105S	inactive mutant enzyme	644016	
3.4.22.53	H262A	inactive mutant enzyme	644016	
3.4.22.53	N286A	inactive mutant enzyme	644016	
3.4.22.53	S369D	inactive mutant enzyme	644047