3.4.22.49	C1129S	inactive	709220	
3.4.22.49	C2029A	-	638869	
3.4.22.49	C2029A	no autoproteolytic cleavage, unable to cleave SCC1	638865	
3.4.22.49	E1483R/R1486E	autoproteolytically cleaved like wild-type	638869	
3.4.22.49	E1503R/R1506E	autoproteolytically cleaved like wild-type	638869	
3.4.22.49	E1532R/R1535E	autoproteolytically cleaved like wild-type	638869	
3.4.22.49	H1531A	active site point mutation prevents Scc1 from being cleaved after binding	638864	
3.4.22.49	additional information	analysis of the homozygous zebrafish mutant cds (cease&desist), bearing a mutation in the separase gene, reveals high levels of polyploidy and aneuploidy, spindle defects, and a mitotic exit delay in mutant embryos. Carcinogenesis studies demonstrate that cds heterozygous adults have an eightfold increase in the percentage of fish bearing epithelial tumors	680013	
3.4.22.49	additional information	clear evidence for a non-proteolytic function of separase is provided: by separase gene deletion in mouse oocytes it is shown that separase null-oocytes are unable to either destroy chesin along chromosome arms or segregate homologous chromosomes and these oocytes are unable to complete cell division. Microinjection of wild-type separase mRNA in separase null-oocytes restores normal homologue disjunction and polar body extrusion	682065	
3.4.22.49	additional information	generation of human cells with one hESP allele-encoding uncleavable protein and another allele harboring a single cleavage site, the cells grow slowly owing to cell cycle delay, in particular during G2/M transition, but not when it was expected, i.e. during anaphase	718194