3.4.22.44 C151A catalytically inactive mutant TVMV protease 718366 3.4.22.44 C151A site-directed mutagenesis, a NIa-Pro active site mutant 718449 3.4.22.44 E189L no self-cleavage 137282 3.4.22.44 E191A site-directed mutagenesis, a VPg-Pro mutant 718449 3.4.22.44 G224R almost no self-cleavage of the 25 kDa protein -, 137276 3.4.22.44 K65A/K67A/C151A catalytically inactive mutant TVMV protease 718366 3.4.22.44 additional information a truncation mutant of TVMV protease lacking the 20 C-terminal amino acid residues, TVMV1-217 protease, shows reduced activity compared to the wild-type enzyme 718366 3.4.22.44 additional information comparison of the catalytic activities of wild-type, mutant and C-terminal truncated proteases -, 137276 3.4.22.44 additional information overexpression of HA-tagged NIa in rat B103 neuroblastoma cells and lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice or human 293T cells, the pattern of NIa expression shows a wide distribution throughout the mouse brain including the cerebral cortex, hippocampus, amygdala, and thalamus, and the Amyloid-beta deposition in the prefrontal cortex, parietal cortex, hippocampus and piriform cortex is remarkably decreased in the brain infused with Lenti-NIa in comparison to the brain infused with Lenti-GFP. Expression of wild-type and utant, with an Asp to Ala substitution in the catalytic triad,.Amyloid-beta intracellularly in B103 cells using the plasmid pGFPUb-Abeta, encoding a triple fusion protein of green fluorescent protein, ubiquitin, and Amylod-beta. The peptide bond between ubiquitin and Amyloid-beta is cleaved quickly by endogenous deubiquitinating enzymes, generating an equimolar ratio of GFP-ubiquitin and Amyloid-beta in the cytosol 718264 3.4.22.44 S129A site-directed mutagenesis, a NIa-Pro phosphorylation-deficient mutant 718449