2.7.2.7	additional information	pta-deleted mutant, BK activity increases by 44%, ack-deleted mutant, similar activity as wild-type	679539	
2.7.2.7	additional information	semi-automated reverse engineering algorithm. The reconstructed metabolic network was used to create a genome-scale model that correctly characterized the butyrate kinase knock-out and the asolventogenic M5 pSOL1 megaplasmid degenerate strains. Systematic gene knock-out simulations performed to identify a set of genes encoding clostridial enzymes essential for growth in silico.	691411	
2.7.2.7	additional information	metabolic engineering studies of Clostridium acetobutylicum strain M5 to produce butanol without acetone	-, 693994	
2.7.2.7	E332D	the mutant shows 3.82% of wild type butyrate kinase II activity	703499	
2.7.2.7	E332L	the mutant shows 0.34% of wild type butyrate kinase II activity	703499	
2.7.2.7	E332Q	the mutant shows 3.02% of wild type butyrate kinase II activity	703499	
2.7.2.7	E334D	the mutant shows 0.29% of wild type butyrate kinase II activity	703499	
2.7.2.7	E334L	the mutant shows0.58% of wild type butyrate kinase II activity	703499	
2.7.2.7	E334Q	the mutant shows 0.41% of wild type butyrate kinase II activity	703499	
2.7.2.7	H155L	the mutant shows no butyrate kinase II activity	703499