2.6.1.98 D156A site-directed mutagenesis, the mutant shows only slightly affected activity and slight loss of binding affinity compared to the wild-type 707534 2.6.1.98 H308A site-directed mutagenesis, the mutant shows only slightly affected activity and slight loss of binding affinity compared to the wild-type 707534 2.6.1.98 K185A site-directed mutagenesis, the activity of the catalytic site mutant is completely abolished 707534 2.6.1.98 additional information construction of a nonpolar knockout of each of wbpA, wbpB, wbpE, wbpD, and wbpI genes. Expression of Bordetella pertussis genes wbpO1629 and wbpO3150 complements a wbpA knockout of Pseudomonas aeruginosa. B-band LPS production is restored to Pseudomonas aeruginosa knockout mutants when the relevant Bordetella pertussis genes are supplied in trans 692874 2.6.1.98 N227A site-directed mutagenesis, the mutant shows only slightly affected activity and slight loss of binding affinity compared to the wild-type 707534 2.6.1.98 Q159A site-directed mutagenesis, the mutant shows only slightly affected activity and slight loss of binding affinity compared to the wild-type 707534 2.6.1.98 R229A site-directed mutagenesis, the mutant shows only slightly affected activity and slight loss of binding affinity compared to the wild-type 707534 2.6.1.98 S180A site-directed mutagenesis, the mutant shows only slightly affected activity and slight loss of binding affinity compared to the wild-type 707534 2.6.1.98 T60A site-directed mutagenesis, the mutant shows only slightly affected activity and slight loss of binding affinity compared to the wild-type 707534 2.6.1.98 Y309A site-directed mutagenesis, the mutant shows only slightly affected activity and slight loss of binding affinity compared to the wild-type 707534