2.4.99.20 D147V mutation has minimal effects on pH-dependence of the enzyme 727857 2.4.99.20 D155N mutation eliminates the strong pH dependence of the catalyzed reactions 727857 2.4.99.20 D155Q mutation eliminates the strong pH dependence of the catalyzed reactions 727857 2.4.99.20 D155Q mutation preserves the strong pH dependence of the catalyzed reactions 727857 2.4.99.20 E146A mutation eliminates the strong pH dependence of the catalyzed reactions 727857 2.4.99.20 E146G mutation eliminates the strong pH dependence of the catalyzed reactions 727857