2.1.1.214	additional information	a plasmid containing a wild-type copy of the TRM112 gene (pBL652) is able to restore the formation of the two modified nucleosides in a trm112-0 strain	712436	
2.1.1.214	additional information	construction of a gene disruptant trm11 (DELTAtrm11) mutant showing the absence of m2s2G10. The lack of 2-methylguanosine (m2G) at position 67 in the trm11 trm14 double disruptant strain suggests that this methylation is mediated by Trm14, which is an m2G6 methyltransferase. The trm11 gene is reinserted into the chiA (Tk1765, chitinase gene) region in the genomic DNA of the DELTAtrm11 strain. Deletion of the Tk1765 gene does not cause growth defects unless chitin is used as a carbon source. Although its expression level is lower in the complemented strain than in the wild-type strain, Trm11 is expressed in the complemented strain. At 85°C, the wild-type, DELTAtrm11, and complemented strains show similar growth curves. As the temperature increases, the growth of the DELTAtrm11 strain is clearly slower than that of the wild-type or complemented strain. At 95°C, the DELTAtrm11 strain shows a considerable growth defect, whereas the complemented strain grows at approximately the same speed as the wild-type strain, indicating that the growth defect of the DELTAtrm11 strain is due to the lack of archaeal Trm11 protein	-, 757106