1.14.14.3 A74F site-directed mutagenesis, the mutant shows reduced activity and increased Km compared to the wild-type enzyme 671985 1.14.14.3 A74G site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme 671985 1.14.14.3 A75G site-directed mutagenesis, activity similar to the wild-type enzyme 657939 1.14.14.3 A75G/C106V/V173A site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum 658060 1.14.14.3 A75G/C106V/V173C site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum 658060 1.14.14.3 A75G/C106V/V173S site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum 658060 1.14.14.3 A75G/C106V/V173T site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum 658060 1.14.14.3 A81H site-directed mutagenesis, residue of the alpha-subunit, mutant shows 13% of wild-type activity 657927 1.14.14.3 alphaDELTA262-290beta four times higher affinity for FMN than wild type 348608 1.14.14.3 alphaF114A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme due to reduced hydrophobicity of the active site 671978