1.14.13.83 C358A no activity, no Fe-S center -, 698972 1.14.13.83 C364A no activity, no Fe-S center -, 698972 1.14.13.83 C394A no activity, no Fe-S center -, 698972 1.14.13.83 C398A no activity, no Fe-S center 698972 1.14.13.83 C42A active, Fe-S center present -, 698972 1.14.13.83 H390A active, Fe-S center present 698972 1.14.13.83 additional information for in vivo enzyme assays an Escherichia coli strain with all necessary genes for the production of cobyric acid except the cobG gene is used for complementation studies in combination with wild-type and mutant cobG genes (site-directed mutagenesis), for in vitro assays an Escherichia coli strain with a coupled multi-enzyme system (plasmid for the production of hydrogenobyrinic acid lacking the cobG gene) is combined with crude extract of an Escherichia coli strain overproducing GobG -, 698972