1.14.11.26 A106T 80% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate 671443 1.14.11.26 A177V random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme -, 744105 1.14.11.26 A311V random mutagenesis 744105 1.14.11.26 C155Y 90% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate 671443 1.14.11.26 C155Y/Y184H/V275I/C281Y 580% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate 671443 1.14.11.26 C281Y 200% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate 671443 1.14.11.26 E16G/T90A/T304A random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme 744105 1.14.11.26 E16G/T90A/T304A site-directed mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme 744105 1.14.11.26 E209Q random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme 744105 1.14.11.26 E82D random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme -, 744105