1.11.1.23	E142A	complete loss of enzymatic activity in vivo and in vitro, 2.1% of the active site iron as compared to wild-type enzyme, no self-hydroxylation at Tyr105	701900	
1.11.1.23	F182A	the variant favors trans-fosfomycin production from (S)-2-hydroxypropylphosphonate	764973	
1.11.1.23	H138A	inactive in vivo, but in vitro it retains 27% of the active site iron and nearly 20% of the wild-type activity, limited self-hydroxylation (at Tyr105)	701900	
1.11.1.23	H180A	complete loss of enzymatic activity in vivo and in vitro, 5.8% of the active site iron as compared to wild-type enzyme, no self-hydroxylation at Tyr105	701900	
1.11.1.23	Y102F	mutant maintains 60% of the activity of the wild-type enzyme	704157	
1.11.1.23	Y103F	mutant maintains 60% of the activity of the wild-type enzyme	704157	
1.11.1.23	Y105F	mutant enzyme is inactive	704157