1.1.1.B52 A158A/N162P 96.8% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion 738052 1.1.1.B52 A158D/N162G 96.5% enantiomeric excess for (R)-3-quinuclidinol production, 87% conversion 738052 1.1.1.B52 A158D/N162Q 95.8% enantiomeric excess for (R)-3-quinuclidinol production, 79% conversion 738052 1.1.1.B52 A158H/N162G/K202Q/L224W mutations increase the enantiomeric excess for (R)-3-quinuclidinol production from 84.3% (wild-type) to 99% and concomitantly to enhance conversion by 43.5% 738052 1.1.1.B52 A158H/N162P 98.1% enantiomeric excess for (R)-3-quinuclidinol production, 95% conversion 738052 1.1.1.B52 A158K/N162M 97.6% enantiomeric excess for (R)-3-quinuclidinol production, 98% conversion 738052 1.1.1.B52 D40A no activity 729146 1.1.1.B52 D40A site-directed mutagenesis, inactive mutant 741816 1.1.1.B52 E197A no activity 729146 1.1.1.B52 E197A site-directed mutagenesis, inactive mutant 741816