4.2.1.18 A240V site-directed mutagenesis, nucleotide exchange C719T 679737 4.2.1.18 E112Q/E132Q/R69A protein is insoluble -, 747539 4.2.1.18 E112Q/E132Q/R69A/Y231F protein is insoluble -, 747539 4.2.1.18 E112Q/E132Q/Y231F protein is insoluble -, 747539 4.2.1.18 E138A inactive mutant enzyme -, 664032 4.2.1.18 E138D the ratio of turnover number to KM-value is 9fold lower than the wild-type ratio -, 664032 4.2.1.18 E138Q the ratio of turnover number to KM-value is 3600fold lower than the wild-type ratio -, 664032 4.2.1.18 G118E no activity with crotonyl-CoA, methylcrotonyl-CoA and methylglutaconyl-CoA -, 664032