3.4.24.55	D79A	no effect on function	667533	
3.4.24.55	E91A	no proteolytic activity	667533	
3.4.24.55	F198A	enzyme remains functional	667533	
3.4.24.55	H193A	enzyme remains functional	667533	
3.4.24.55	K197A	enzyme remains functional	667533	
3.4.24.55	additional information	heterologous expression of enzyme in yeast, enzyme functionally substitutes for yeast Ax11p and Ste23p in pheromone production. Independent heterologous expression of N-terminal domain with amino acids 1-515 or C-terminal domain with amino acids 516-962 does not promote yeast mating. Co-expression of both domains restores yeast mating	667533	
3.4.24.55	N119A	promotion of yeast mating factor in heterologous expression is disabled	667533	
3.4.24.55	N317A/R318A/S319A	enzyme remains functional	667533	
3.4.24.55	R792A	no proteolytic activity	667533	
3.4.24.55	R792A/T793A/E794A/E795A	no proteolytic activity	667533	
3.4.24.55	S117A	enzyme remains functional	667533	
3.4.24.55	S117A/H118A/N119A	promotion of yeast mating factor in heterologous expression is disabled	667533	
3.4.24.55	S196A/K197A/F198A/S199A	promotion of yeast mating factor in heterologous expression is disabled	667533	
3.4.24.55	T127A	enzyme remains functional	667533	
3.4.24.55	T322A/L323A	enzyme remains functional	667533	
3.4.24.55	Y799A	no proteolytic activity	667533