3.4.22.55	D316A/D330A	abolishes auto-processing and reduces enzymatic activity dramatically, 840fold decrease in activity	664882	
3.4.22.55	additional information	caspase prodomain-DELTA25 mutant is slightly less effective than wild-type caspase-2, inducing cell death of about 70% of transfected cells. Caspase prodomain-DELTA25 mutant cannot kill due to its impaired nuclear localization	647762	
3.4.22.55	C320A	catalytic residue, inactive mutant enzyme	713154	
3.4.22.55	C303A	catalytically inactive	665588	
3.4.22.55	C320A	catalytically inactive	732048	
3.4.22.55	S139An	expression of mutant S139A leads to significantly enhanced growth rate of cells	732048	
3.4.22.55	C320A	forms a dimer only when cell are treated with DRB	664753	
3.4.22.55	D316A	fusion of the linker to the large subunit, toxic when expressed in yeast	664882	
3.4.22.55	D330A	fusion of the linker to the small subunit, slightly greater deleterious effect on enzyme activity than fusion to the large subunit (D316A), toxic when expressed in yeast	664882	
3.4.22.55	C303A	inactive	664882	
3.4.22.55	additional information	knockdown of caspase-2 and overexpression of a caspase-2 mutant	717457	
3.4.22.55	K152A	mutant enzyme with strikingly altered caspase-2 localization. Whereas caspase-2 characteristically accumulates in the nucleus forming dots or filaments, the mutant enzyme is mostly localized outside and exclusively of the nucleus forming dot-like aggregates. K152A mutants can also kill transfected cells at comparable levels to the wild-type version	647762	
3.4.22.55	S157A/C320A	nonphosphorylatable, dimerizes constitutively	664753	
3.4.22.55	S135A	phosphorylation site	711848	
3.4.22.55	S340A	phosphorylation site, expression of mutant S340A more than doubles the sensitivity of cells to nocodazole-induced mitotic death compared with wild-type protein	711848	
3.4.22.55	S308A	phosphorylation site, S308 phosphorylated in mitosis, abrogates mitotic suppression, processing of mutant S308A in mitosis does not occur immediately, but rather occurs with roughly the same kinetics as wild-type caspase-2 in interphase	711848	
3.4.22.55	S135A	phosphorylation site, suppressed equally compared with wild type protein during mitosis	711848	
3.4.22.55	D152A	pro-caspase mutant, like the wild-type, this mutant is efficiently processed between the large and the small subunit, however, it is not further processed to seperate the prodomain from the large subunit	664882	
3.4.22.55	S135A/S308A	rapidly processed in mitosis compared with caspase-2 S308A mutant	711848	
3.4.22.55	T380A	site-directed mutagenesis	717846	
3.4.22.55	T380A/Y420A	site-directed mutagenesis	717846	
3.4.22.55	Y420A	site-directed mutagenesis	717846	
3.4.22.55	D316G	uncleavable	665588