3.4.22.10	C192S	inactive	680146, 681702	
3.4.22.10	C192S	inactive mutant	-, 667311, 667426, 668911, 670124	
3.4.22.10	C192S	lacks protease activity	680145	
3.4.22.10	C192S	no autocatalytic processing of the 40000 Da zymogen to the 28000 Da mature form, no proteolytic activity	81463	
3.4.22.10	C47S	crystal structure analysis	668772	
3.4.22.10	C47S	inactive mutant	698880	
3.4.22.10	C47S	site-directed mutagenesis, inactive mutant	-, 670312	
3.4.22.10	D9N	protease activity (microgram/min/mg) substrate azocasein: 256, substrate proSPE B C47S mutant: 250, compared to wild-type 341 and 378, respectively	698880	
3.4.22.10	G136A	protease activity (microgram/min/mg) substrate azocasein: 22.3, substrate proSPE B C47S mutant: 8.1, compared to wild-type 341 and 378, respectively	698880	
3.4.22.10	G163S	protease activity (microgram/min/mg) substrate azocasein: 348, substrate proSPE B C47S mutant: 385, compared to wild-type 341 and 378, respectively	698880	
3.4.22.10	G163S/A172S	protease activity (microgram/min/mg) substrate azocasein: 286, substrate proSPE B C47S mutant: 236, compared to wild-type 341 and 378, respectively	698880	
3.4.22.10	G239D	protease activity (microgram/min/mg) substrate azocasein: 35.3, substrate proSPE B C47S mutant: 20, compared to wild-type 341 and 378, respectively	698880	
3.4.22.10	G308S	behaviour is similar to the wild type enzyme	680146	
3.4.22.10	G378A	Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type	-, 734185	
3.4.22.10	G380A	Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type	-, 734185	
3.4.22.10	G381A	Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type	-, 734185	
3.4.22.10	G382A	Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type	-, 734185	
3.4.22.10	G384A	Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type	734185	
3.4.22.10	G384D	Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) decreased compared to wild-type and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) 50% decreased compared to wild-type	-, 734185	
3.4.22.10	G385A	Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) minimally increased compared to wild-type and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) 3fold increased	734185	
3.4.22.10	additional information	construction of an isogenic inactive mutant strain M6	670124	
3.4.22.10	T379A	Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) slightly decreased compared to wild-type	734185	
3.4.22.10	T379V	Km(acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) and kcat (acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin) slightly decreased compared to wild-type	734185	
3.4.22.10	V189A	protease activity (microgram/min/mg) substrate azocasein: 2.6, substrate proSPE B C47S mutant: 0.9, compared to wild-type 341 and 378, respectively	698880	
3.4.22.10	W212A	protease activity (microgram/min/mg) substrate azocasein: 1.2, substrate proSPE B C47S mutant: 0.9, compared to wild-type 341 and 378, respectively	698880	
3.4.22.10	W214A	protease activity (microgram/min/mg) substrate azocasein: 0.8, substrate proSPE B C47S mutant: 0.8, compared to wild-type 341 and 378, respectively	698880