3.4.15.6	additional information	coexpression of cyanophycin and CGPase in the same plant without substrate degradation in planta by transient expression of CGPase CphB either in the plastid or cytosol, or the Pseudomonas alcaligenes CGPase CphE in the cytosol of CGP-producing Nicotiana tabacum plants. No cyanophycin degradation appears prior to cell homogenization independent of the CGPase produced. In crude plant extracts, only cytosolic CphE leads to a fast degradation of cyanophycin. CphE also shows higher stability and in vitro activity compared to both CphB variants	755615	
3.4.15.6	additional information	Gly101, Asp172, Gly173, Arg178, Arg180 and Arg183 form a conserved pocket adjacent to the catalytic Ser132, these are functionally critical residues. The cyanophycinase active site contains three absolutely conserved Arg residues (Arg178, Arg180 and Arg183), which line up along one side of the pocket	709525	
3.4.15.6	additional information	optimized transient expression in Nicotiana benthamiana plants to produce high amounts of active CGPase. Protein stability is increased by the translational fusion of CGPase to the green fluorescent protein or to the transit peptide of the small subunit of RuBisCO for peptide production in the chloroplasts	755028	
3.4.15.6	N100A	site-directed mutagenesis, 40% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525	
3.4.15.6	N158A	site-directed mutagenesis, 41% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525	
3.4.15.6	N172A	site-directed mutagenesis, 0.02% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525	
3.4.15.6	N17A	site-directed mutagenesis, 60% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525	
3.4.15.6	N202A	site-directed mutagenesis, 8% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525	
3.4.15.6	Q101A	site-directed mutagenesis, 0.02% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525	
3.4.15.6	Q173A	site-directed mutagenesis, 0.5% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525	
3.4.15.6	R178A	site-directed mutagenesis, 1% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525	
3.4.15.6	R180A	site-directed mutagenesis, 0.01% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525	
3.4.15.6	R183a	site-directed mutagenesis, 0.01% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525	
3.4.15.6	S132A	site-directed mutagenesis, 0.002% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C	709525