3.2.2.8	H239A	dramatic increase in Km for uridine, unchanged kcat	657373	
3.2.2.8	H82N	small increase in Km, increase in kcat	657373	
3.2.2.8	additional information	mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination	700741	
3.2.2.8	Q227A	the mutation causes an increase of kcat for uridine and inosine	696258	
3.2.2.8	Q227F	the mutation causes an increase of kcat for uridine and inosine	696258	
3.2.2.8	Q227Y	the mutation has a strong, enhancing effect on the hydrolysis of inosine, and the catalytic efficiency for the purinic substrate is increased by a factor of 7.6	696258	
3.2.2.8	T223A	the mutation does not improve significantly the catalytic efficiency of YeiK toward inosine	696258	
3.2.2.8	T223F	the mutation does not improve significantly the catalytic efficiency of YeiK toward inosine	696258	
3.2.2.8	T223F/Q227Y	the mutant shows a 2fold increase in catalytic efficiency toward inosine	696258	
3.2.2.8	T223Y	the mutation does not affect the specificity of the enzyme toward inosine or uridine	696258	
3.2.2.8	T223Y/Q227Y	contrary to wild-type, mutant is able to process inosine	751203	
3.2.2.8	T223Y/Q227Y	the mutant displays a catalytic efficiency toward inosine that is more than 50fold increased compared to that of wild type enzyme	696258	
3.2.2.8	T227A	the mutation does not improve significantly the catalytic efficiency of YeiK toward inosine	696258	
3.2.2.8	T227F	the mutation does not improve significantly the catalytic efficiency of YeiK toward inosine	696258