3.1.4.55	D164A	0.07% of wild-type activity	721652	
3.1.4.55	D187A	83% of wild-type activity	721652	
3.1.4.55	D54A	0.09% of wild-type activity	721652	
3.1.4.55	D80A	0.07% of wild-type activity	721652	
3.1.4.55	D80A	inactive with substrate bis(p-nitrophenyl)phosphate	719851	
3.1.4.55	H200A	10% of wild-type activity, 6.33fold decrease in kcat/KM with substituted methyl phenylphosphate diesters with leaving group pKa values ranging from 4 to 8.4. With bis(pnitrophenyl)phosphate as a substrate, there is a 10fold decrease in kcat/KM, primarily the result of a large increase in KM. The nickel ion-activated H200A mutant displays a bell-shaped pH dependence for kcat/KM with pKa values comparable to those of the wild-type enzyme	721652	
3.1.4.55	H222A	14% of wild-type activity	721652	
3.1.4.55	H78A	0.3% of wild-type activity	721652	
3.1.4.55	T75A	3% of wild-type activity	721652