2.7.2.7 additional information metabolic engineering studies of Clostridium acetobutylicum strain M5 to produce butanol without acetone -, 693994 2.7.2.7 additional information pta-deleted mutant, BK activity increases by 44%, ack-deleted mutant, similar activity as wild-type 679539 2.7.2.7 additional information semi-automated reverse engineering algorithm. The reconstructed metabolic network was used to create a genome-scale model that correctly characterized the butyrate kinase knock-out and the asolventogenic M5 pSOL1 megaplasmid degenerate strains. Systematic gene knock-out simulations performed to identify a set of genes encoding clostridial enzymes essential for growth in silico. 691411 2.7.2.7 H183L the mutant shows 0.06% of wild type butyrate kinase II activity 703499 2.7.2.7 H183Q the mutant shows 0.19% of wild type butyrate kinase II activity 703499 2.7.2.7 E334D the mutant shows 0.29% of wild type butyrate kinase II activity 703499 2.7.2.7 E332L the mutant shows 0.34% of wild type butyrate kinase II activity 703499 2.7.2.7 R215F E332D the mutant shows 0.38% of wild type butyrate kinase II activity 703499 2.7.2.7 E334Q the mutant shows 0.41% of wild type butyrate kinase II activity 703499 2.7.2.7 R215K the mutant shows 0.44% of wild type butyrate kinase II activity 703499 2.7.2.7 R150K the mutant shows 21% of wild type butyrate kinase II activity 703499 2.7.2.7 E332Q the mutant shows 3.02% of wild type butyrate kinase II activity 703499 2.7.2.7 E332D the mutant shows 3.82% of wild type butyrate kinase II activity 703499 2.7.2.7 H155L the mutant shows no butyrate kinase II activity 703499 2.7.2.7 H155Q the mutant shows no butyrate kinase II activity 703499 2.7.2.7 R150F the mutant shows no butyrate kinase II activity 703499 2.7.2.7 E334L the mutant shows0.58% of wild type butyrate kinase II activity 703499