2.3.1.250	C187A	site-directed mutagenesis, increase in Wnt signaling activity of about 1.5fold upon expression of PORCNC187A compared to wild-type PORCN	734745	
2.3.1.250	C375X	site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 resi­due, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant	734745	
2.3.1.250	C381X	site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 resi­due, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant	734745	
2.3.1.250	C385X	site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 resi­due, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant	734745	
2.3.1.250	H341A	site-directed mutagenesis, catalytic active site mutant, Wnt signaling inactive mutant	734194	
2.3.1.250	H341A	site-directed mutagenesis, catalytically inactive mutant	734745	
2.3.1.250	H341A	site-directed mutagenesis, the mutation reduces Wnt signaling relative to wild-type PORCN	734745	
2.3.1.250	H341D	site-directed mutagenesis, catalytically inactive mutant	733699	
2.3.1.250	H341Q	site-directed mutagenesis, catalytically inactive mutant	733699	
2.3.1.250	additional information	generation of three different deletion mutants of the enzyme lacking residues 1-34, 1-66, or 1-110 (DELTA34, DELTA66, and DELTA110), the mutants show affeced N-glycosylation of Wingless protein, because the mutant enzymes cannot bind to Wingless, overview	733769	
2.3.1.250	additional information	identification of enzyme mutants with a 219-kb deletion in Xp11.23, heterozygous and mosaic mutations in gene PORCN, genotyping, overview	734754	
2.3.1.250	additional information	overexpression of HA-tagged human PORC to confirm the ability of hPORC RNAi constructs to knockdown hPORC expression	733699	
2.3.1.250	additional information	PORCN is deleted by adenoviral Cre-GFP excision in immortalized MEF cells, PORCN null cells cannot activate WNT3A	734194	
2.3.1.250	additional information	rescue of Drosophila melanogaster porc embryos by Mporc RNA injection	733769	
2.3.1.250	additional information	rescue of Drosophila porc embryos by Mus musculus Mporc RNA injection	733769	
2.3.1.250	additional information	the catalytically inactive porcupine is able to act as an inhibitor of endogenous porcupine, possibly via direct competition for substrate	733699	
2.3.1.250	additional information	the cysteine mutations might be detrimental to the enzyme's tertiary structure and its catalytic activity	734745	
2.3.1.250	additional information	zinc finger nuclease-mediated PORCN gene deletion in HT1080 cells. Mutation or skipping of PORCN exon 9 creates cells null for PORCN catalytic activity	734194