2.3.1.169	A110C	absence of strong cooperative inhibition of CO which characterizes wild-type enzyme	655801	
2.3.1.169	A110C	mutant designed to block the CO-migrating tunnel in the alpha-subunit. Electron paramagnetic resonance spectra indicates that the A-cluster is properly assembled. ACS activity is similar to that of the wild-type recombinant Ni-activated alpha-subunit	687824	
2.3.1.169	A219F	mutant designed to block the tunnel through which CO and CO2 migrate. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed	674925	
2.3.1.169	A222L	mutant designed to block the CO-migrating tunnel in the alpha-subunit. Electron paramagnetic resonance spectra indicates that the A-cluster is properly assembled. ACS activity is similar to that of the wild-type recombinant Ni-activated alpha-subunit	687824	
2.3.1.169	A222L	the bifunctional mutant enzyme is not able to synthesize acetyl-CoA using CO2 as substrate	655801	
2.3.1.169	A265M	absence of strong cooperative inhibition of CO which characterizes wild-type enzyme	655801	
2.3.1.169	A578C	mutant designed to block the tunnel through which CO and CO2 migrate. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed	674925	
2.3.1.169	F70W	mutant designed to block the region that connects the tunnel at the betabeta interface with a water channel also located at the interface. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed	674925	
2.3.1.169	L215F	mutant designed to block the tunnel through which CO and CO2 migrate. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed	674925	
2.3.1.169	additional information	construction of several cdh gene knockouts, genotypic and phenotypic analysis of cdh mutants. CODH activity during aceticlastic and carboxidotrophic growth is reduced in the cdh1 mutant (strain MCD1) compared to the wild-type	719744	
2.3.1.169	N101Q	mutant designed to block the region that connects the tunnel at the betabeta interface with a water channel also located at the interface. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed	674925