2.1.1.264	D195A	site-directed mutagenesis, the mutation does not affect the methylation activity	720598	
2.1.1.264	D568A	site-directed mutagenesis, the D568A mutation in the C-terminal domain does not rescue m7G2069 formation, but m2G2445 is efficiently formed in this mutant	720598	
2.1.1.264	D597A	site-directed mutagenesis, the mutation does not affect the methylation activity	720598	
2.1.1.264	additional information	generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY	-, 720601	
2.1.1.264	N309A	site-directed mutagenesis, the N309A mutation in the NTD impairs m2G2445 formation, but rescues m7G2069 formation	720598	
2.1.1.264	N397A	site-directed mutaagenesis, the N397A mutant exhibits no m2G2445 formation but rescues m7G2069 formation	720598	
2.1.1.264	R530A	site-directed mutagenesis, the mutation does not affect the methylation activity	720598