1.14.13.50 F223A site-directed mutagenesis, increased activity compared to the wild-type enzyme 658439 1.14.13.50 F225A site-directed mutagenesis, increased activity compared to the wild-type enzyme 658439 1.14.13.50 F85A site-directed mutagenesis, 60% decreased activity compared to the wild-type enzyme 658439 1.14.13.50 F85A site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme 658210 1.14.13.50 F87A site-directed mutagenesis, mutant enzyme is nearly not soluble in contrast to the wild-type, highly reduced activity compared to the wild-type enzyme 658439 1.14.13.50 moe construction of an allelic exchange mutant and a pcpD knockout mutant strains, the latter show reduced activity at high pentachlorophenol concentrations, not at low concentrations 659022 1.14.13.50 additional information introduction of tobacco etch virus protease cleavage site into the enzyme as a glutathione-S-transferase fusion protein 438820 1.14.13.50 additional information targeted mutagenesis via allelic exchange, construction of isogenic mutant without PCP 4-monooxygenase activity and 2 cointegration mutants with slow activity 438819 1.14.13.50 R235A site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme 658210 1.14.13.50 R235E site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme 658210 1.14.13.50 R235E site-directed mutagenesis, reduced activity compared to the wild-type enzyme 658439 1.14.13.50 R235K site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme 658210 1.14.13.50 R235K site-directed mutagenesis, increased activity compared to the wild-type enzyme 658439 1.14.13.50 R235K site-directed mutagenesis, mutant enzyme is nearly not soluble in contrast to the wild-type, reduced activity compared to the wild-type enzyme 658439 1.14.13.50 Y216A site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme 658210 1.14.13.50 Y216A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme 658439 1.14.13.50 Y216F site-directed mutagenesis, 60% decreased activity compared to the wild-type enzyme 658439 1.14.13.50 Y397A site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme 658210 1.14.13.50 Y397A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme 658439 1.14.13.50 Y397F site-directed mutagenesis, 60% increased activity compared to the wild-type enzyme 658439 1.14.13.50 Y397F site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme 658210