1.14.12.22	degradation	constitutive expression of terminal oxygenase subunit in Rhizobium tropici. In water-cultured siratro rhizospheres inoculated with the recombinant strain, 48% of the initial 25 microg dibenzofuran is removed within 3 days, and similar results are obtained in soil-cultured siratro rhizosperes using sterile vermiculite. In non-sterile field soils, the inoculated recombinant strain can grow on the siratro root in all soils tested, except for wet paddy field	-, 707774	
1.14.12.22	F275A	mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene	707776	
1.14.12.22	F275I	mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene	707776	
1.14.12.22	F275L	mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene	707776	
1.14.12.22	F275V	mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene	707776	
1.14.12.22	F275W	mutation in oxygenase component. Decrease in activity with carbazole, wild-type like activity with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene	707776	
1.14.12.22	F275W	the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme	741682	
1.14.12.22	F329A	mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene	707776	
1.14.12.22	F329I	mutation in oxygenase component. Strong decrease in activity with all substrates tested	707776	
1.14.12.22	F329L	mutation in oxygenase component. Strong decrease in activity with all substrates tested	707776	
1.14.12.22	F329V	mutation in oxygenase component. Strong decrease in activity with all substrates tested	707776	
1.14.12.22	F329W	mutation in oxygenase component. Strong decrease in activity with all substrates tested	707776	
1.14.12.22	I262A	mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene	707776	
1.14.12.22	I262L	mutation in oxygenase component. Increase in activity with anthracene	707776	
1.14.12.22	I262V	mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene	707776	
1.14.12.22	I262V	the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme	741682	
1.14.12.22	I262W	mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene	707776	
1.14.12.22	additional information	expression of the catalytic subunit CarAa is enhanced by induction at a lower cell concentration and temperature and over a longer time, while the expression of subunits CarAc and CarAd is inverse. Best co-expression condition tested is cell concentration at induction of 0.5 (absorbance at 600 nm), 37°C amd 4 h	725637	
1.14.12.22	Q282N	mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene	707776	
1.14.12.22	Q282S	mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene	707776	
1.14.12.22	Q282Y	mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene	707776	
1.14.12.22	Q282Y	the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme	741682