1.13.11.50	E69Q	lower thermal stability of beta-sheet secondary structure, half catalytic center activity and remarkably silent difference in apparent substrate binding compared to the wild type enzyme	675466	
1.13.11.50	E98A	site-direted mutagenesis	711267	
1.13.11.50	E98Q	an interplay of residues Glu98, His104, Glu11 (from the neighbor subunit), and Arg80 is the most important for the Fe2+ transport in and out of the protein	742322	
1.13.11.50	E98Q	site-direted mutagenesis	711267	
1.13.11.50	F115A	site-directed mutagenesis, the mutant shows reduced turnover and altered iron binding compared to the wild-type enzyme	725553	
1.13.11.50	F119A	site-directed mutagenesis, the mutant shows reduced turnover and altered iron binding compared to the wild-type enzyme	725553	
1.13.11.50	F59A	site-directed mutagenesis, the mutant shows reduced turnover and altered iron binding compared to the wild-type enzyme	725553	
1.13.11.50	H104E	no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions	696107	
1.13.11.50	H104N	approximately 1% of the specific activity of wild-type enzyme with substrate pentan-2,4-dione, retains binding affinity for Fe2+ at binding site I, binding seems to be tighter than in wild-type, small effect on other metal ions	696107	
1.13.11.50	H62E	no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions	696107	
1.13.11.50	H62N	no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, binding disruption of Cu2+, Mn2+, and Ni2+ compared with wild-type	696107	
1.13.11.50	H64D	no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions	696107	
1.13.11.50	H64E	no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions	696107	
1.13.11.50	H64N	conversion of substrate in a strictly Fe2+-concentration dependent manner, substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions	696107	
1.13.11.50	additional information	the exchange of 3 histidines in the Fe2+-binding centre shows that these histidines are crucial for for binding Fe2+ and for Fe2+-dependent dioxygenase activity	696107	
1.13.11.50	R80A	mutation causes relocation of Glu11 from neighbor subunit closer to His104, enabling formation of the Glu11(OE1/2)-His104(NE2H/CD2H) H-bond	742322	
1.13.11.50	R80A	site-direted mutagenesis	711267	
1.13.11.50	R80A/E98A	site-direted mutagenesis	711267	
1.13.11.50	T107A	site-direted mutagenesis	711267	
1.13.11.50	Y70A	an interplay of residues Glu98, His104, Glu11 (from the neighbor subunit), and Arg80 is the most important for the Fe2+ transport in and out of the protein	742322	
1.13.11.50	Y70A	site-direted mutagenesis	711267	
1.13.11.50	Y70A/R80A/E98A	site-direted mutagenesis	711267	
1.13.11.50	Y70F	site-directed mutagenesis, the mutant shows reduced turnover and altered iron binding compared to the wild-type enzyme	725553	
1.13.11.50	Y70F	site-direted mutagenesis	711267	
1.13.11.50	Y70F/R80A/E98A	site-direted mutagenesis	711267