1.1.1.B52	K202S/L224Y	89.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion	738052	
1.1.1.B52	K202N/L224M	89.7% enantiomeric excess for (R)-3-quinuclidinol production, 90% conversion	738052	
1.1.1.B52	N162A	93.8% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion	738052	
1.1.1.B52	K202R/L224W	94.7% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion	738052	
1.1.1.B52	K202Q/L224W	95.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion	738052	
1.1.1.B52	A158D/N162Q	95.8% enantiomeric excess for (R)-3-quinuclidinol production, 79% conversion	738052	
1.1.1.B52	A158D/N162G	96.5% enantiomeric excess for (R)-3-quinuclidinol production, 87% conversion	738052	
1.1.1.B52	A158A/N162P	96.8% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion	738052	
1.1.1.B52	A158K/N162M	97.6% enantiomeric excess for (R)-3-quinuclidinol production, 98% conversion	738052	
1.1.1.B52	A158H/N162P	98.1% enantiomeric excess for (R)-3-quinuclidinol production, 95% conversion	738052	
1.1.1.B52	Y216V	activity is 31% compared to activity of wild-type enzyme	729146	
1.1.1.B52	R196A	activity is 76% compared to activity of wild-type enzyme	729146	
1.1.1.B52	A158H/N162G/K202Q/L224W	mutations increase the enantiomeric excess for (R)-3-quinuclidinol production from 84.3% (wild-type) to 99% and concomitantly to enhance conversion by 43.5%	738052	
1.1.1.B52	D40A	no activity	729146	
1.1.1.B52	E197A	no activity	729146	
1.1.1.B52	D40A	site-directed mutagenesis, inactive mutant	741816	
1.1.1.B52	E197A	site-directed mutagenesis, inactive mutant	741816	
1.1.1.B52	R196A	site-directed mutagenesis, the mutant shows 34% reduced activity compared to the wild-type enzyme	741816	
1.1.1.B52	Y216V	site-directed mutagenesis, the mutant shows 69% reduced activity compared to the wild-type enzyme	741816